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Laboratory Safety Rules Observations and study of structure of microbes Microbial physiology: growth and metabolism Bacterial genetics and molecular biology Environment microbilogy Medical microbiology & immunology Control of microbial activities Reagents Stains Media Buffers and Solutions
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Microorganisms exist in a large and mixed population and therefore isolation of microorganisms to pure cultures will be the initial step in the study of the characteristics and potential of microorganisms. In nature, microbial populations do not segregate themselves by species but exist with a mixture of many other cell types. In the laboratory, these populations can be separated into pure cultures. These cultures contain only one type of organism and are suitable for the study of their cultural, morphological, and biochemical properties. In this experiment, you will first use one of the techniques designed to produce discrete colonies. Colonies are individual, macroscopically visible masses of microbial growth on a solid medium surface, each representing the multiplication of a single organism. Once you have obtained these discrete colonies, you will make an aseptic transfer onto nutrient agar slants for the isolation of pure cultures. Streak Plate : Isolation of Discrete Colonies from a Mixed Culture PRINCIPLE: The techniques commonly used for isolation of discrete colonies initially require that the number of organisms in the inoculum be reduced. The resulting diminution of the population size ensures that, following inoculation, individual cells will be sufficiently far apart on the surface of the agar medium to effect a separation of the different species present. The following are techniques that can be used to accomplish this necessary dilution: 1. The streak-plate method is a rapid qualitative isolation method. It is essentially a dilution technique that involves spreading a loopful of culture over the surface of an agar plate.
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